- Sharon Lipinski, Christine Forster
- 5,746.4 metre(s)
- United States (Loveland)
The longest line of toothbrushes is 5,746.4 m (18,853 ft 0.22 in), and was achieved by Sharon Lipinski and Christine Forster (both USA) in Loveland, Colorado, USA, on 26 February 2019.
This record was attempted to raise awareness for children's oral health and they used 41,769 individually wrapped toothbrushes.