- Sharon Lipinski, Christine Forster
- 41,769 total number
- United States (Loveland)
The longest line of toothbrushes consist of 41,769 toothbrushes, and was achieved by Sharon Lipinski and Christine Forster (both USA) in Loveland, Colorado, USA, on 26 February 2019.
This record was attempted to raise awareness for children's oral health and the final line measured 5,746.4 m (18,853 ft 0.22 in).