https://kids.guinnessworldrecords.com en

First assassination of a head of state by firearm

First assassination of a head of state by firearm
Who
James Stewart, the 1st Earl of Moray and Regent of, James Hamilton of Bothwellhaugh, Scotland
What
First
Where
Not Applicable
When
23 January 1570
The first assassination of a head of state by firearm was carried out on 23 January 1570, when James Hamilton (of Bothwellhaugh, Scotland) used a carbine to fire on James Stewart, the 1st Earl of Moray and Regent of Scotland for his nephew, the child King James VI of Scotland. The assassination occurred during Stewart's visit to Linlithgow – a historic Royal Burgh located in the central lowlands of Scotland. James Hamilton was a fervent supporter of Mary Queen of Scots. Hamilton fatally wounded James Stewart with a carbine shot from a window of his uncle Archbishop Hamilton's house. The Regent's body was shipped to Leith then taken to Holyrood Abbey. Moray was buried on 14 February 1570 in St. Anthony's aisle at St. Giles Kirk in Edinburgh.

This was also the first assassination of any kind by firearm.