- Gaylord Palms Resort & Convention Center
- 448 pair(s)
- United States (Kissimmee)
The most couples kissing under the mistletoe at a single venue is 448, and was achieved by Gaylord Palms Resort & Convention Center (USA) in Kissimmee, Florida, USA, on 7 December 2017.
The attempt took place in the hotel's St. Augustine atrium, and all participants received a sprig of real mistletoe to kiss beneath.