- United Kingdom (Manea)
The largest litter of puppies is 24, all of whom were born on 29 November 2004 to Tia, a Neopolitan mastiff, owned by Damian Ward (UK) and Anne Kellegher (Ireland) of Manea, Cambridgeshire, UK.
They were born by Caesarian section, one was still born, three died in the first week. There were nine females, 15 males in total. Tia's full name is Abellatino Arabella. The father is called Caesar.