Most comic book characters on a single issue cover
Who
G.I.Joe
What
236 total number
Where
Not Applicable
When

The cover of G. I. Joe: America's Elite #25 (Devil's Due Publishing) features 236 characters (not counting animals/pets such as Order the German Shepherd, Max the Bobcat or Spirit the Eagle). Issued in May 2007, the "July" issue cover was drawn by Chris Lie.